Part:BBa_K3978049:Design

reget-plasmid-ER

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 112
  Illegal EcoRI site found at 5428
  Illegal XbaI site found at 30
  Illegal XbaI site found at 7054
  Illegal XbaI site found at 8110
  Illegal XbaI site found at 8479
  Illegal PstI site found at 131
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 112
  Illegal EcoRI site found at 5428
  Illegal PstI site found at 131
  Illegal NotI site found at 149
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 112
  Illegal EcoRI site found at 5428
  Illegal BglII site found at 305
  Illegal BglII site found at 5704
  Illegal BglII site found at 7789
  Illegal BglII site found at 7834
  Illegal BglII site found at 7882
  Illegal BglII site found at 8065
  Illegal BglII site found at 8101
  Illegal BamHI site found at 106
  Illegal XhoI site found at 354
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 112
  Illegal EcoRI site found at 5428
  Illegal XbaI site found at 30
  Illegal XbaI site found at 7054
  Illegal XbaI site found at 8110
  Illegal XbaI site found at 8479
  Illegal PstI site found at 131
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 112
  Illegal EcoRI site found at 5428
  Illegal XbaI site found at 30
  Illegal XbaI site found at 7054
  Illegal XbaI site found at 8110
  Illegal XbaI site found at 8479
  Illegal PstI site found at 131
  Illegal NgoMIV site found at 324
  Illegal NgoMIV site found at 671
  Illegal NgoMIV site found at 5348
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 7949
  Illegal BsaI.rc site found at 1256
  Illegal SapI.rc site found at 2916
  

Design Notes

ER is originally expressed in eukaryotic cells. Codon optimization is needed for expression in E. coli. The expression of ER in prokaryotes is small.

Source

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